Combined IL-15/IL-15Rα Immunotherapy Maximizes IL-15 Activity In Vivo

Combined IL-15/IL-15R Immunotherapy Maximizes IL-15 Activity In Vivo

Soluble IL-15/IL-15Rα complexes in human serum.

In this issue of Blood, Bergamaschi and colleagues demonstrate that effectively all circulating IL-15 found in the serum of humans and mice is associated with the soluble IL-15Rα.(1)

Transpresentation of interleukin-15 by IL-15/IL-15Rα mRNA-engineered human dendritic cells boosts antitumoral natural killer cell activity.

In cancer immunotherapy, the use of dendritic cell (DC)-based vaccination strategies can improve overall survival, but until now durable clinical responses remain scarce. To date, DC vaccines are designed primarily to induce effective T-cell responses, ignoring the antitumor activity potential of natural killer (NK) cells. Aiming to further improve current DC vaccination outcome, we engineered ...

Electrotransfer of IL-15/IL-15Rα complex for the treatment of established melanoma

Background Interleukin-15 (IL-15) mediates the function of some innate and adaptive immune cells, which makes it an attractive target as an anti-cancer agent. Gene electrotransfer (GET) has been used to safely deliver plasmid DNA (pDNA) to tissues in a number of pre-clinical and clinical studies for cancer therapy. Previously we have shown using GET to deliver interleukin-15 (IL-15) to mouse me...

Circulating IL-15 exists as heterodimeric complex with soluble IL-15Rα in human and mouse serum.

IL-15 is an important cytokine for the function of the immune system, but the form(s) of IL-15 produced in the human body are not fully characterized. Coexpression of the single-chain IL-15 and the IL-15 receptor alpha (IL-15Rα) in the same cell allows for efficient production, surface display, and eventual cleavage and secretion of the bioactive IL-15/IL-15Rα heterodimer in vivo, whereas the s...